Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
PBMC
Tissue
blood
Lineage
mesoderm
Description
peripheral blood mononuclear cells

Attributes by original data submitter

Sample

source_name
hPBMC
gender
male
tissue
Blood
cell type
PBMC
chip antibody
anti-human RUNX1 antibody (Abcam, cat.no ab23980)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and RUNX1 or TET2-DNA complexes were isolated with antibody. ChIPed DNA was subjected to the library construction with KAPA Library Preparation Kit (KAPA Biosystems, Imc.,MA, USA) according to the manufacture’s instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
30736227
Reads aligned (%)
57.8
Duplicates removed (%)
33.6
Number of peaks
63733 (qval < 1E-05)

hg38

Number of total reads
30736227
Reads aligned (%)
58.9
Duplicates removed (%)
32.8
Number of peaks
64350 (qval < 1E-05)

Base call quality data from DBCLS SRA