GSM2686885: SET-2 pSTAT1-2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Leukemia Acute Myelogenous
Attributes by original data submitter
SET2 cells were grown in RPMI-1640 supplemented with 20% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin.
Sequenced DNA Library
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries for Illumina sequencing were prepared using the KAPA Hyper Prep Kit for Illumina and ligated to unique Bioo Scientific NEXTflex barcode adaptors. Following ligation, libraries were amplified with 8-12 cycles of PCR and were then size-selected using a 2% gel cassette in the Pippin Prep System from Sage Science. DNA fragments of size 200-500bp were captured. Libraries were quantified by qPCR utilizing the KAPA Biosystems Illumina Library Quantification kit.