Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
JURKAT
cell line
JURKAT
cell type
human T cell leukemia cells
antibody
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H3K27Ac ChIP: 10 million cells were used for the ChIP and precipitated using antibody against human H3K27Ac (Cell Signaling Technology, 8173S, lot #1). Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
52213715
Reads aligned (%)
98.6
Duplicates removed (%)
6.1
Number of peaks
960 (qval < 1E-05)

hg19

Number of total reads
52213715
Reads aligned (%)
97.5
Duplicates removed (%)
8.0
Number of peaks
912 (qval < 1E-05)

Base call quality data from DBCLS SRA