Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
primary HUVEC cells
treatment
no treat
experiment
ChIP-seq
antibody
H3K27ac (Abcam, ab4729)
restriction enzyme
no

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hi-C experiments were conducted in biological replicates using HindIII according to previous publication (Lieberman-Aiden, E. et al. Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science 326, 289-93 (2009).). ChIP-seq protocol can be found from http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf. GRO-seq experiments were performed according to previous publication (Wang, D. et al. Reprogramming transcription by distinct classes of enhancers functionally defined by eRNA. Nature 474, 390-4 (2011).). Libraries were prepared according to Illumina's instruction or cited literatures.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
34468373
Reads aligned (%)
85.1
Duplicates removed (%)
17.0
Number of peaks
55320 (qval < 1E-05)

hg19

Number of total reads
34468373
Reads aligned (%)
84.8
Duplicates removed (%)
17.3
Number of peaks
55390 (qval < 1E-05)

Base call quality data from DBCLS SRA