Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Lung

Cell type

Lung

MeSH Description

Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Sample

source_name

Lung

strain

C57BL/6

tissue

Lung

chip antibody

Pol II CTD (4H8) Sc-47701 SantaCruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Hearts, kidneys, livers and lungs were recovered, flash frozen and stored at -80oC. Tissues were crosslinked and sheared. Fragmented chromtin was used in ChIP with Pol II antibody. Indexed libraries were constructed using Tn5 transposase tagmentations, as per Nextera protocol (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

43433827

Reads aligned (%)

33.3

Duplicates removed (%)

16.2

Number of peaks

97 (qval < 1E-05)

mm9

Number of total reads

43433827

Reads aligned (%)

33.3

Duplicates removed (%)

16.2

Number of peaks

86 (qval < 1E-05)