Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Lung

Cell type

NCI-H1299

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma Non-Small Cell

Sample

source_name

H1299

cell line

NIH H1299

cell type

non-small cell lung cancer

treatment

control vector

antibody

Abcam, Ab8580

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq were performed following the protocol: Using ChIP-seq technology to generate high-resolution profiles of histone modifications. O'Geen H, Echipare L, Farnham PJ. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. Samples were sequenced using the Illumina Solexa Hiseq 2500.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

51912408

Reads aligned (%)

94.9

Duplicates removed (%)

40.0

Number of peaks

26121 (qval < 1E-05)

hg19

Number of total reads

51912408

Reads aligned (%)

94.7

Duplicates removed (%)

40.3

Number of peaks

26150 (qval < 1E-05)