GSM2677923: MCF7 shKMT2C 2 H3K4me1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 cells
cell type
metastatic ER+ breast cancer
antibody
H3K4me1 antibody (abcam 8895)
cell line
MCF7
knockdown
shKMT2C
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using 5ng of DNA and KAPA Hyper Prep Kit for Illumina, according to the manufacturer’s protocol. Libraries were validated using the Agilent 2200 Tapestation and KAPA library quantification qPCR assay