Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Blood
Cell type
J-Lat A72
NA
NA

Attributes by original data submitter

Sample

source_name
J-Lat A72
treatment
JQ1
chip antibody
Abcam ab110641

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was extracted using the QIAquick PCR Purification Kit (Qiagen). RNA was isolated via RNeasy Plus Mini Kit (Qiagen). ChIP libraries were generate using the Ovation Ultralow Library Systems V2 (NuGEN). ATAC libraries were generated using the Nextera DNA Sample Prep Kit (Illumina). RNA libraries were generated using the Ovation RNA-Seq System V2 (NuGEN).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
46280996
Reads aligned (%)
176.1
Duplicates removed (%)
8.4
Number of peaks
2416 (qval < 1E-05)

hg38

Number of total reads
46280996
Reads aligned (%)
177.2
Duplicates removed (%)
8.2
Number of peaks
2622 (qval < 1E-05)

Base call quality data from DBCLS SRA