Antigen

Antigen Class

TFs and others

Antigen

TP53

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7

passage

between 4-14 for all experiments

antibody

X-DO1, Santa Cruz sc-126 X

cell line

MCF7

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-Seq, cells were crosslinked in 1% formaldehyde and quenched with glycine after 5min, and collected by scraping after washes in PBS+ protease inhibitors. ChIP seq libraries were prepared using Nugen Ovation Ultralow Library V2 systems kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

30311015

Reads aligned (%)

97.8

Duplicates removed (%)

10.8

Number of peaks

1944 (qval < 1E-05)

hg19

Number of total reads

30311015

Reads aligned (%)

97.2

Duplicates removed (%)

11.8

Number of peaks

1945 (qval < 1E-05)