GSM2671292: p53 ChIP-Seq, t=2.5h, IR 10Gy; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP53
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7
passage
between 4-14 for all experiments
antibody
X-DO1, Santa Cruz sc-126 X
cell line
MCF7
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, cells were crosslinked in 1% formaldehyde and quenched with glycine after 5min, and collected by scraping after washes in PBS+ protease inhibitors. ChIP seq libraries were prepared using Nugen Ovation Ultralow Library V2 systems kit