GSM2670873: SUM44 Input LTED; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
SUM 44PE
Primary Tissue
Breast
Tissue Diagnosis
Unknown
Attributes by original data submitter
Sample
source_name
breast cancer cell line
tissue
breast cancer cell line
biomaterial provider
ATCC
cell line
SUM44
estrogen
No, LTED (long term estrogen deprived)
antibody
none
esr1 mutation
Y537S
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in 1% formaldehyde at room temperature for 10 minutes and then quenched with 125 mmol/L glycine. Samples were then lysed, sonicated, and chromatin was immunoprecipitated by overnight incubation at 4C with ER antibodies prebound with Protein G magnetic dynabeads (Invitrogen). Samples were then washed vigorously with RIPA buffer and reversed crosslinked by an overnight incubation in elution buffer at 65C. DNA was digested with RNase and Proteinase K, purify precipitated with phenol chloroform and eluted in Tris-HCl pH 8.0.