Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PGR

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D cells
tissue type
T47D cells
er/pr/her2 status
ER+ PR+
drug treatment
10 nM R5020
hormone exposure time
45 mins
protocol
ChIP-seq
antibody
anti-phospho-PR Ser294 (from Dean Edwards, Baylor College of Medicine)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq) of the paper.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
5557193
Reads aligned (%)
85.9
Duplicates removed (%)
14.0
Number of peaks
6035 (qval < 1E-05)

hg38

Number of total reads
5557193
Reads aligned (%)
86.3
Duplicates removed (%)
13.5
Number of peaks
6021 (qval < 1E-05)

Base call quality data from DBCLS SRA