GSM2125171: MCF7 E2+Dex 75 mins (15 min E2 and 60 min E2+Dex) INPUT rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Breast cancer cells
tissue type
ER+/PR+ MCF7 cell models
er/pr status
ER+/PR+
drug treatment
10nM Estradiol + 10 nM Dexamethasone
hormone exposure time
75 minutes (15 min E2 and 60 min E2+Dexamethasone)
chip antibody
none (INPUT)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method (ChIP and ChIP-sequencing). ChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq) of the paper.