Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Breast cancer cells
tissue type
ER+/PR+ MCF7 cell models
er/pr status
ER+/PR+
drug treatment
10 nM DHT
hormone exposure time
45 minutes
chip antibody
none (INPUT)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method (ChIP and ChIP-sequencing). ChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq) of the paper.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
40382328
Reads aligned (%)
99.0
Duplicates removed (%)
10.9
Number of peaks
910 (qval < 1E-05)

hg19

Number of total reads
40382328
Reads aligned (%)
98.1
Duplicates removed (%)
12.2
Number of peaks
1065 (qval < 1E-05)

Base call quality data from DBCLS SRA