Antigen

Antigen Class

TFs and others

Antigen

HIC1

Cell type

Cell type Class

Blood

Cell type

iTreg

NA

NA

Sample

source_name

human iTreg cells

tissue

cord blood

cell type

iTreg cells

antibody

HIC-1 antibody (Santa Cruz, sc-271499)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and HIC1 protein-DNA complexes were isolated with antibody. The DNA libraries were prepared as per the guidelines from Illumina by Fasteris Life Sciences (Plan-les-Ouates, Switzerland). Input DNA was sequenced and used as a control. DNA libraries were se-quenced on Illumina HiSeq2500 producing from 25 to 35 million reads per sample. The 50 nucleotide reads were aligned to hg19 build of human reference genome using bowtie2 (version 2.2.9)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31025336

Reads aligned (%)

91.3

Duplicates removed (%)

34.8

Number of peaks

12484 (qval < 1E-05)

hg19

Number of total reads

31025336

Reads aligned (%)

90.9

Duplicates removed (%)

35.6

Number of peaks

12584 (qval < 1E-05)