Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Human Embryonic Kidney cells
cell line
HEK293
passages
p20
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were disrupted using LB3 buffer (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% NaDeoxycholate, 0.5% N-lauroylsarcosine) Illumina TruSeq DNA Sample Preparation Kit v2 (Illumina, San Diego)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
40357665
Reads aligned (%)
95.1
Duplicates removed (%)
8.9
Number of peaks
1308 (qval < 1E-05)

hg19

Number of total reads
40357665
Reads aligned (%)
94.2
Duplicates removed (%)
10.2
Number of peaks
1384 (qval < 1E-05)

Base call quality data from DBCLS SRA