Antigen

Antigen Class

TFs and others

Antigen

Suz12

Cell type

Cell type Class

Blood

Cell type

Th17 Cells

MeSH Description

A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Sample

source_name

Primary CD4+ Th cells

cell type

Activated CD4+ T cells skewed towards a Th17 subset.

strain

C57BL/6

genotype

Jarid2fl/fl; CD4cre

chip antibody

Suz12 (Cell Signaling, D39F6)

chip antibody manufacturer

Cell Signaling

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

In brief, 10 million Th17 cells were fixed, lysed and sonicated to 200 base pairs. Sheared chromatin was submitted to Peconic LLC (http://www.peconicgenomics.com/) along with antibodies for ChIP-exo.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

3761457

Reads aligned (%)

72.4

Duplicates removed (%)

82.2

Number of peaks

43 (qval < 1E-05)

mm9

Number of total reads

3761457

Reads aligned (%)

72.3

Duplicates removed (%)

82.6

Number of peaks

45 (qval < 1E-05)