Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 cells
cell line
MCF7
er/pr status
ER+/PR+
drug treatment
Vehicle
hormone exposure time
4 hours
protocol
Cell models ChIP-seq
chip antibody
anti-ER (SC biotech HC-20, cat# SC-543, lot# L1911)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method (ChIP and ChIP-sequencing) ChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq) of the paper.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
28302544
Reads aligned (%)
70.2
Duplicates removed (%)
9.5
Number of peaks
12297 (qval < 1E-05)

hg38

Number of total reads
28302544
Reads aligned (%)
71.9
Duplicates removed (%)
7.9
Number of peaks
12375 (qval < 1E-05)

Base call quality data from DBCLS SRA