Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
ESD3 mouse embryonic stem cells
cell fate transition type
Cellular Reprogramming
time-point
N/A
antibody
N/A
sorting
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
29329158
Reads aligned (%)
96.8
Duplicates removed (%)
6.4
Number of peaks
166 (qval < 1E-05)

mm9

Number of total reads
29329158
Reads aligned (%)
96.7
Duplicates removed (%)
6.9
Number of peaks
178 (qval < 1E-05)

Base call quality data from DBCLS SRA