Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
Mouse Embryonic Fibroblasts
cell fate transition type
Cellular Reprogramming
time-point
Day 0
antibody
N/A
sorting
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
32549123
Reads aligned (%)
98.5
Duplicates removed (%)
7.8
Number of peaks
183 (qval < 1E-05)

mm9

Number of total reads
32549123
Reads aligned (%)
98.3
Duplicates removed (%)
8.4
Number of peaks
172 (qval < 1E-05)

Base call quality data from DBCLS SRA