Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Input control for WT spleen CD4+ Tcells sample
cell type
spleen CD4+ T cells
source
spleen
strain
C57BL/6
chip antibody
no antibody added

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and WASp-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
12658673
Reads aligned (%)
99.6
Duplicates removed (%)
5.2
Number of peaks
198 (qval < 1E-05)

mm9

Number of total reads
12658673
Reads aligned (%)
99.5
Duplicates removed (%)
5.2
Number of peaks
210 (qval < 1E-05)

Base call quality data from DBCLS SRA