Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse Embryonic Stem Cells (mESCs)
strain/background
C57bl/6
mll2 status
WT
cell type
Embryonic stem cells
chip antibody
IgG (Abcam, 172730)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq: ChIP experiments were carried out following a standard protocol in the lab (Morey et al., Cell Stem Cell 2013). RNAseq: RNA was extracted using RNeasy kit (Qiagen). Libraries were prepared according to Illumina instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
48901849
Reads aligned (%)
95.8
Duplicates removed (%)
51.6
Number of peaks
621 (qval < 1E-05)

mm9

Number of total reads
48901849
Reads aligned (%)
95.6
Duplicates removed (%)
51.6
Number of peaks
655 (qval < 1E-05)

Base call quality data from DBCLS SRA