Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mouse Embryonic Stem Cells (mESCs)

strain/background

C57bl/6

mll2 status

KO

cell type

Embryonic stem cells

chip antibody

H3 (Abcam, ab1791)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPseq: ChIP experiments were carried out following a standard protocol in the lab (Morey et al., Cell Stem Cell 2013). RNAseq: RNA was extracted using RNeasy kit (Qiagen). Libraries were prepared according to Illumina instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

89049470

Reads aligned (%)

98.0

Duplicates removed (%)

19.6

Number of peaks

720 (qval < 1E-05)

mm9

Number of total reads

89049470

Reads aligned (%)

97.7

Duplicates removed (%)

19.5

Number of peaks

817 (qval < 1E-05)