Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

Jurkat (Clone E6-1)

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

Control shRNA+TNF stim

tissue origin

Peripherial Blood

cell line

E6-1

cell type

T lymphocyte supension

infected with

lentiviral vector expressing control shRNA

stimulated with

10ng.ml TNF-a for 30min

chip antibody

rabbit-anti-RNA Pol II (N-20) antibody

chip antibody vendor

Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked with formaldehyde, washed twice with phosphate buffered saline and and nuclei isolated with hypotonic buffer and centrifugation. EMBO J. 25, 3596–3604. Samples were immunoprecipitated with rabbit-anti-RNA Pol II (N-20) antibody from Santa Cruz (catalog # sc-899). Illumina library preparation standard protocol

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

77884760

Reads aligned (%)

94.2

Duplicates removed (%)

11.2

Number of peaks

34445 (qval < 1E-05)

hg19

Number of total reads

77884760

Reads aligned (%)

93.4

Duplicates removed (%)

11.7

Number of peaks

34303 (qval < 1E-05)