GSM1150961: Control shRNA-unstim; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
Jurkat (Clone E6-1)
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
Control shRNA-unstim
tissue origin
Peripherial Blood
cell line
E6-1
cell type
T lymphocyte supension
infected with
lentiviral vector expressing control shRNA
stimulated with
none (unstimulated control)
chip antibody
rabbit-anti-RNA Pol II (N-20) antibody
chip antibody vendor
Santa Cruz
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde, washed twice with phosphate buffered saline and and nuclei isolated with hypotonic buffer and centrifugation. EMBO J. 25, 3596–3604. Samples were immunoprecipitated with rabbit-anti-RNA Pol II (N-20) antibody from Santa Cruz (catalog # sc-899). Illumina library preparation standard protocol