Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA3

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D
cell line
T47D wt/R330fsl cells
passages
<10
antibody
anti-GATA3 C-terminus (ThermoFisher, PA5-20892)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to BIOO SCIENTIFIC instruction. ATAC-seq libraries were prepared by following the original protocol (Buenrostro et al., 2013) with several modifications (as described above)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
39031379
Reads aligned (%)
164.2
Duplicates removed (%)
11.7
Number of peaks
3739 (qval < 1E-05)

hg38

Number of total reads
39031379
Reads aligned (%)
166.4
Duplicates removed (%)
11.4
Number of peaks
4119 (qval < 1E-05)

Base call quality data from DBCLS SRA