Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D
cell line
T47D Flp control cells
passages
<10
antibody
anti-ERalpha (Santa Cruz, HC-20)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to BIOO SCIENTIFIC instruction. ATAC-seq libraries were prepared by following the original protocol (Buenrostro et al., 2013) with several modifications (as described above)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
50297783
Reads aligned (%)
96.1
Duplicates removed (%)
49.0
Number of peaks
10124 (qval < 1E-05)

hg38

Number of total reads
50297783
Reads aligned (%)
98.3
Duplicates removed (%)
48.3
Number of peaks
10561 (qval < 1E-05)

Base call quality data from DBCLS SRA