Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Ptulid_NCoRKO_WCE

strain background

C57BL/6J

genotype/variation

NCoR1 KO

diet

PTU/LID

tissue

liver

chip antibody

NA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were sonicated and histone-DNA complexes were isolated with antibody. After performing reverse cross linking on sheared chromatin the following steps were performed: end-repair, addition of A base to 3' end of DNA fragments, ligation of adapters to ends of DNA fragments, enrichment by PCR, and gel purification of PCR products.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

46829191

Reads aligned (%)

98.2

Duplicates removed (%)

10.9

Number of peaks

254 (qval < 1E-05)

mm9

Number of total reads

46829191

Reads aligned (%)

98.1

Duplicates removed (%)

12.0

Number of peaks

272 (qval < 1E-05)