Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
Chow_NCoRKO_WCE
strain background
C57BL/6J
genotype/variation
NCoR1 KO
diet
Chow
tissue
liver
chip antibody
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated and histone-DNA complexes were isolated with antibody. After performing reverse cross linking on sheared chromatin the following steps were performed: end-repair, addition of A base to 3' end of DNA fragments, ligation of adapters to ends of DNA fragments, enrichment by PCR, and gel purification of PCR products.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
43956183
Reads aligned (%)
98.3
Duplicates removed (%)
10.2
Number of peaks
249 (qval < 1E-05)

mm9

Number of total reads
43956183
Reads aligned (%)
98.2
Duplicates removed (%)
11.3
Number of peaks
274 (qval < 1E-05)

Base call quality data from DBCLS SRA