600 wing imaginal discs were dissected (taking one disc per larva) in Schneider medium and aliquoted per 50 in 1.5 mL microtubes on ice. Discs were fixed at 22°C. 12 sonication cycles were performed (Diagenode Bioruptor sonifier; cycles of 30'' ON, 30'' OFF, high power). After centrifugation, the 12 supernatants were pooled, homogenized, and 2% were removed (Input). The remaining fragmented chromatin was redistributed into 12 tubes and each tube was adjusted to 1 mL with 140 mM NaCl, 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% BSA, Roche complete EDTA-free protease inhibitor cocktail). For immunoprecipitation, 3 µg of anti-Myc antibody (Abcam 9132) were added per tube. The of water. Two biological replicates were performed. Library preparation and Illumina sequencing were performed at the Ecole normale superieure Genomic Platform (Paris, France). Libraries were prepared using NEXTflex ChIP-Seq Kit (Bioo Scientific), using 38 ng of IP or Input DNA. Libraries were multiplexed by 10 on one flowcell run. A 75 bp single read sequencing was performed on a NextSeq 500 device (Illumina).