Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

Commercially available hepatocytes (BioreclamationIVT: lot QSK)

individual

d6

cell type

Primary human hepatocytes

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were sonicated and histone-DNA complexes were isolated with antibody. After performing reverse cross linking on sheared chromatin the following steps were performed: end-repair, addition of A base to 3' end of DNA fragments, ligation of adapters to ends of DNA fragments, enrichment by PCR, and gel purification of PCR products.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

47703550

Reads aligned (%)

96.9

Duplicates removed (%)

3.7

Number of peaks

2085 (qval < 1E-05)

hg19

Number of total reads

47703550

Reads aligned (%)

95.8

Duplicates removed (%)

4.2

Number of peaks

556 (qval < 1E-05)