Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Digestive tract

Cell type

HCT-8

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Cultured HCT8 INPUT control

cell line

HCT8

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Illumina TruSeq™ DNA Sample Preparation Twenty five microliters of immunoprecipitated, FAIRE or input DNA was used for library preparation. For CHIP samples Illumina (San Diego, CA) TruSeq™ DNA Sample Preparation reagents were used, with ligation of 1/10th the manufacturers recommended adapter amounts and agarose gel selection of DNA fragments in the 200-500 bp size range. For FAIRE samplesThruPLEX DNA-Seq reagents was used (Rubicon Genomics, Ann Arbor, MI, USA), following manufacturer’s instructions”.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

44297286

Reads aligned (%)

99.7

Duplicates removed (%)

68.9

Number of peaks

843 (qval < 1E-05)

hg19

Number of total reads

44297286

Reads aligned (%)

99.1

Duplicates removed (%)

70.5

Number of peaks

1010 (qval < 1E-05)