Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293T cells

cell line

HEK293T (ATCC CRL-3216)

transfected with

None

chip antibody

H3K4me3 (Abcam ab8580)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as described in Gupta-Hinch et al. 2014 Libraries were prepared for sequencing using standard Illumina protocols by the Oxford Genomics Centre.

Sequencing Platform

instrument_model

Illumina HiSeq 1000

hg38

Number of total reads

65908260

Reads aligned (%)

99.5

Duplicates removed (%)

9.5

Number of peaks

26982 (qval < 1E-05)

hg19

Number of total reads

65908260

Reads aligned (%)

99.1

Duplicates removed (%)

9.5

Number of peaks

29768 (qval < 1E-05)