Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kdm4a

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
B220+ B cells
strain background
C57BL/6
tissue
spleen
cell type
splenic B220+ B cells
genotype/variation
Tg(IghelMD4)4Ccg
chip antibody
anti-Jmjd2a (abcam, ab70786)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were be prepared by the EZ-ChIP kit (EMD Millipore, Cat. #: 17-371), sequentially sonicated to obtain fragmanted DNA, then complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions by using the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
36055982
Reads aligned (%)
81.4
Duplicates removed (%)
17.4
Number of peaks
599 (qval < 1E-05)

mm9

Number of total reads
36055982
Reads aligned (%)
81.1
Duplicates removed (%)
17.4
Number of peaks
646 (qval < 1E-05)

Base call quality data from DBCLS SRA