Antigen

Antigen Class

TFs and others

Antigen

DNMT3B

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC HUES8

NA

NA

Sample

source_name

HUES8 human embryonic stem cells (hESCs)

cell line

HUES8 hESCs

genotype

Inactivating mutations in TET1, TET2 and TET3

antibody

DNMT3B

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq was performed for TET1 (WT hESCs) and DNMT3B (WT and TKO hESCs). WT and TKO hESCs were cultured in standard hESC media, as described above. ~5 × 107 cells were fixed, washed and snap-frozen according to the Cell Fixation protocol from Active Motif (http://www.activemotif.com/documents/1848.pdf). Immunoprecipitation and library prep were performed by Active Motif Epigenetic Services (https://www.activemotif.com/catalog/704/epigenetic-services)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

31153334

Reads aligned (%)

95.2

Duplicates removed (%)

6.5

Number of peaks

5481 (qval < 1E-05)

hg19

Number of total reads

31153334

Reads aligned (%)

94.9

Duplicates removed (%)

6.7

Number of peaks

5637 (qval < 1E-05)