GSM2642522: HUES8 WT TET1 ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TET1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC HUES8
NA
NA
Attributes by original data submitter
Sample
source_name
HUES8 human embryonic stem cells (hESCs)
cell line
HUES8 hESCs
genotype
Wild type sequence
antibody
TET1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq was performed for TET1 (WT hESCs) and DNMT3B (WT and TKO hESCs). WT and TKO hESCs were cultured in standard hESC media, as described above. ~5 × 107 cells were fixed, washed and snap-frozen according to the Cell Fixation protocol from Active Motif (http://www.activemotif.com/documents/1848.pdf). Immunoprecipitation and library prep were performed by Active Motif Epigenetic Services (https://www.activemotif.com/catalog/704/epigenetic-services)