Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TET1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC HUES8
NA
NA

Attributes by original data submitter

Sample

source_name
HUES8 human embryonic stem cells (hESCs)
cell line
HUES8 hESCs
genotype
Wild type sequence
antibody
TET1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq was performed for TET1 (WT hESCs) and DNMT3B (WT and TKO hESCs). WT and TKO hESCs were cultured in standard hESC media, as described above. ~5 × 107 cells were fixed, washed and snap-frozen according to the Cell Fixation protocol from Active Motif (http://www.activemotif.com/documents/1848.pdf). Immunoprecipitation and library prep were performed by Active Motif Epigenetic Services (https://www.activemotif.com/catalog/704/epigenetic-services)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
58266690
Reads aligned (%)
28.5
Duplicates removed (%)
68.6
Number of peaks
2438 (qval < 1E-05)

hg38

Number of total reads
58266690
Reads aligned (%)
29.3
Duplicates removed (%)
67.9
Number of peaks
2507 (qval < 1E-05)

Base call quality data from DBCLS SRA