Antigen

Antigen Class

TFs and others

Antigen

Rela

Cell type

Cell type Class

Blood

Cell type

Treg

NA

NA

Sample

source_name

Treg unstimulated

mouse strain

C57BL/6

tissues

Spleens+Lymph nodes

cell type

Regulatory T cells

cell subtype

Treg unstimulated

chip antibody

anti-mouse p65 (Santa Cruz, sc-372, #K-0615)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After nuclear isolation, nuclear lysates were sonicated by using bioruptor followed by immunoprecipitating with anti-p65 antibody+ Dynabeads protein A Library was constructed on immunoprecipitated DNA according to manufacturer's recommendations (KAPA Hyper Prep Kit). Samples were enriched with PCR with 12 cycles.Afterward, double size selection was performed with AMPure XP beads

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

32147804

Reads aligned (%)

82.4

Duplicates removed (%)

20.6

Number of peaks

3189 (qval < 1E-05)

mm9

Number of total reads

32147804

Reads aligned (%)

82.3

Duplicates removed (%)

20.5

Number of peaks

3146 (qval < 1E-05)