GSM2641055: p65 IP in Treg, unstim, rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Rela
Cell type
Cell type Class
Blood
Cell type
Treg
NA
NA
Attributes by original data submitter
Sample
source_name
Treg unstimulated
mouse strain
C57BL/6
tissues
Spleens+Lymph nodes
cell type
Regulatory T cells
cell subtype
Treg unstimulated
chip antibody
anti-mouse p65 (Santa Cruz, sc-372, #K-0615)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After nuclear isolation, nuclear lysates were sonicated by using bioruptor followed by immunoprecipitating with anti-p65 antibody+ Dynabeads protein A Library was constructed on immunoprecipitated DNA according to manufacturer's recommendations (KAPA Hyper Prep Kit). Samples were enriched with PCR with 12 cycles.Afterward, double size selection was performed with AMPure XP beads