Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Treg and Tconv
NA
NA

Attributes by original data submitter

Sample

source_name
Treg + Tconv stim + unstim input chromatin
mouse strain
C57BL/6
tissues
Spleens+Lymph nodes
cell type
Treg+Tconv
cell subtype
Treg + Tconv stim + unstim input chromatin
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After nuclear isolation, nuclear lysates were sonicated by using bioruptor followed by immunoprecipitating with anti-p65 antibody+ Dynabeads protein A Library was constructed on immunoprecipitated DNA according to manufacturer's recommendations (KAPA Hyper Prep Kit). Samples were enriched with PCR with 12 cycles.Afterward, double size selection was performed with AMPure XP beads

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
38927348
Reads aligned (%)
96.1
Duplicates removed (%)
8.4
Number of peaks
169 (qval < 1E-05)

mm9

Number of total reads
38927348
Reads aligned (%)
95.9
Duplicates removed (%)
8.4
Number of peaks
143 (qval < 1E-05)

Base call quality data from DBCLS SRA