Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Biotin

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Pro-B cell
strain
C57BL/6
genotype
Yy1(ihCd2/+ ) Rag2(-/-) R26(BirA/BirA)
cell type
Pro-B cell
primer
Dynabeads M-280 Streptavidin (Invitrogen)
antibody
Dynabeads M-280 Streptavidin (Invitrogen)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
About 5x107 pro-B cells were used for chromatin precipitation by streptavidin pulldown (Bio-ChIP) Ebert et al., 2011 library construction protocol: The precipitated genomic DNA was quantified by real-time PCR, and at least 5 ng of ChIP-precipitated DNA were used as starting material for the generation of paired-end sequencing libraries as described by Illumina’s ChIP Sequencing sample preparation protocol followed by size selection of DNA fragments of 200–350 bp. Completed libraries were quantified with the Agilent Bioanalyzer dsDNA 1000 assay kit and Agilent QPCR NGS library quantification kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
25486075
Reads aligned (%)
96.8
Duplicates removed (%)
21.6
Number of peaks
9887 (qval < 1E-05)

mm9

Number of total reads
25486075
Reads aligned (%)
96.7
Duplicates removed (%)
21.8
Number of peaks
9850 (qval < 1E-05)

Base call quality data from DBCLS SRA