Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NOTCH1

Cell type

Cell type Class
Cardiovascular
Cell type
Aortic valve endothelial cells
NA
NA

Attributes by original data submitter

Sample

source_name
aortic valve endothelial cells
cell type
Primary aortic valve endothelial cells
passage
p8
chip antibody
anti-myc tag (myc-Notch)
chip antibody manufacturer
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was isolated by phenol/chloroform/isoamylalcohol. ChIPs were done in triplicate with Protein G magnetic beads and then pooled for subsequent ChIP-seq library preparation with the standard Illumina ChIP-seq library protocol.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
29707270
Reads aligned (%)
86.5
Duplicates removed (%)
10.6
Number of peaks
1261 (qval < 1E-05)

hg38

Number of total reads
29707270
Reads aligned (%)
88.6
Duplicates removed (%)
9.3
Number of peaks
998 (qval < 1E-05)

Base call quality data from DBCLS SRA