GSM2640419: hT2-2D/FOXA1 Ipt ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pancreas
Cell type
Pancreatic ductal adenocarcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
hT2-2D/FOXA1 cells
strain
PDA patient
cell type
Monolayer-cultured cells established from PDX-derived primary tumor organoid
peturbation
Transduced with MSCV/FOXA1 retrovirus
antibody
Ipt genomic DNA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from ~1 millon single cell dissociated of organoid cultures, or ~5 million of monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared according to Illumina's instructions of TruSeq ChIP Sample Prep kit.