Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pancreas
Cell type
Pancreatic ductal adenocarcinoma
NA
NA

Attributes by original data submitter

Sample

source_name
hT1 organoid
strain
PDA patient
cell type
Patient-derived PDA primary tumor organoid (hT1)
antibody
Ipt genomic DNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from ~1 millon single cell dissociated of organoid cultures, or ~5 million of monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared according to Illumina's instructions of TruSeq ChIP Sample Prep kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
63255019
Reads aligned (%)
98.8
Duplicates removed (%)
3.1
Number of peaks
1439 (qval < 1E-05)

hg19

Number of total reads
63255019
Reads aligned (%)
97.8
Duplicates removed (%)
4.8
Number of peaks
1427 (qval < 1E-05)

Base call quality data from DBCLS SRA