Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pancreas

Cell type

Pancreatic ductal adenocarcinoma

NA

NA

Sample

source_name

T6/sgp53 organoid

strain

C57BL/6

cell type

Cas9-expressing mouse PDA primary tumor organoid (T6-Cas9)

peturbation

Transduced with sgp53 lentivirus

antibody

Ipt genomic DNA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared from ~1 millon single cell dissociated of organoid cultures, or ~5 million of monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared according to Illumina's instructions of TruSeq ChIP Sample Prep kit.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

152272475

Reads aligned (%)

98.6

Duplicates removed (%)

33.7

Number of peaks

763 (qval < 1E-05)

mm9

Number of total reads

152272475

Reads aligned (%)

98.3

Duplicates removed (%)

33.7

Number of peaks

906 (qval < 1E-05)