Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

Three-week cultured Th2 cells generated from primary splenic CD4_T cells

cell type

Th2 cells

strain

C57BL/6

chip antibody

none

genotype/variation

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Antibody-specific immunoprecipitates and total input DNA samples were prepared using a NEBNext ChIP-Seq Library Prep Reagent Set for Illumina. Adaptor-ligated DNA was recovered using AMPure XP Beads. This DNA was then amplified by 15 cycles of PCR and again recovered using AMPure XP Beads. Fifty cycles of sequencing reaction were preformed on an Illumina HiSeq1500.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

26740688

Reads aligned (%)

98.3

Duplicates removed (%)

25.5

Number of peaks

602 (qval < 1E-05)

mm9

Number of total reads

26740688

Reads aligned (%)

98.0

Duplicates removed (%)

25.6

Number of peaks

685 (qval < 1E-05)