GSM2636315: SH-SY5Y 5azaC SmcHD1 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMCHD1
Cell type
Cell type Class
Neural
Cell type
SH-SY5Y
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
SH-SY5Y neuroblastoma-5azaC treated
cell line
SH-SY5Y
cell line source tissue
bone marrow
cell type
neurobalstoma cells
passage
15-20
treated with
DNA demethylating agent 5azaC for 72 hrs
chip antibody
anti-SmcHD1 antiserum
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were fixed using 1% formaldehyde. Upon cell cysis, the chromatin was sheared by sonication and SmcHD1 bound chromatin was isolated using anti-SmcHD1 antiserum. The DNA libraries were prepared according the Illumina's suggested protocol.