Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOS

Cell type

Cell type Class
Bone
Cell type
MNNG
NA
NA

Attributes by original data submitter

Sample

source_name
highly metastatic osteosarcoma cell line
cell line
MNNG
chip antibody
rabbit anti-c-Fos (Santa Cruz #sc-52)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols. ChIP-Seq: 36bp single end reads (MG63 and MG63.3), 50bp single end reads (all other samples); RNA-seq: 100bp paired-end reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
65035170
Reads aligned (%)
91.9
Duplicates removed (%)
5.1
Number of peaks
1844 (qval < 1E-05)

hg38

Number of total reads
65035170
Reads aligned (%)
94.3
Duplicates removed (%)
3.5
Number of peaks
2108 (qval < 1E-05)

Base call quality data from DBCLS SRA