Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
Drosophila S2 cells
tissue
embryonic
cell type
S2
antibody
none
treatment
KDM4ARNAi

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
chromatin crosslinking with 1% paraformaldehyde for 10 minutes, shearing with Bioruptor sonicator (30 cycles), immunoprecipitation with anti-H3K9me3 or anti-H3K36me3 antibodies with Protein-A Dynabeads standard Illumina TruSeq protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
12923525
Reads aligned (%)
94.5
Duplicates removed (%)
20.2
Number of peaks
5557 (qval < 1E-05)

dm3

Number of total reads
12923525
Reads aligned (%)
95.0
Duplicates removed (%)
17.4
Number of peaks
4990 (qval < 1E-05)

Base call quality data from DBCLS SRA