Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

Drosophila S2 cells

tissue

embryonic

cell type

S2

antibody

H3K36Me3

treatment

KDM4ARNAi

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

chromatin crosslinking with 1% paraformaldehyde for 10 minutes, shearing with Bioruptor sonicator (30 cycles), immunoprecipitation with anti-H3K9me3 or anti-H3K36me3 antibodies with Protein-A Dynabeads standard Illumina TruSeq protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

9804058

Reads aligned (%)

98.1

Duplicates removed (%)

9.2

Number of peaks

5448 (qval < 1E-05)

dm3

Number of total reads

9804058

Reads aligned (%)

98.2

Duplicates removed (%)

8.5

Number of peaks

5488 (qval < 1E-05)