Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL11A

Cell type

Cell type Class
Blood
Cell type
Pre-B cell leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
peripheral blood pre-B cell leukemia
cell type
peripheral blood pre-B cell leukemia
chip antibody
Anti-BCL11a polyclonal (Bethyl Labs BL1797)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
5593402
Reads aligned (%)
48.9
Duplicates removed (%)
0.6
Number of peaks
40 (qval < 1E-05)

hg19

Number of total reads
5593402
Reads aligned (%)
48.4
Duplicates removed (%)
0.6
Number of peaks
41 (qval < 1E-05)

Base call quality data from DBCLS SRA